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KMID : 0380020050200050338
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Korean Journal of Biotechnology and Bioengineering
2005 Volume.20 No. 5 p.338 ~ p.340
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In-vitro Refolding of PEGylated Lipase
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Kim Min-Young
Kwon Jin-Sook
Lee Eun-Kyu
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Abstract
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Covalent modification of a protein with polyethylene glycol (PEG) has become one of the most widely used and well established drug enhancement strategies in the biopharmaceutical industry. The general benefits enjoyed by PEGylation, such as prolonged serum half-lives or reduced immunogenicity in vivo, are well known. By now the PEGylation process has been performed with purified proteins, and it is required to recover the desired PEGylate by a multi-step purification process. The ultimate aim of our research is to develop an integrated process of PEGylation and in vitro refolding starting with inclusion body material. For this, we investigated the feasibility that a protein could be PEGylated under a denaturing condition and also the PEGylated proteins could be refolded correctly. Using lipase as a model protein, we found that it was PEGylated in the presence of 8M urea and that the PEG molecules covalently attached to lipase did not appear to hinder its refolding.
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KEYWORD
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PEGylated Lipase, PEGylation, refolding, integrated process
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